Folate receptor beta as an imaging target in myocardial infarction
Anand, T M Arman Hossain (2023)
Anand, T M Arman Hossain
2023
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2023050340465
https://urn.fi/URN:NBN:fi-fe2023050340465
Tiivistelmä
For decades, myocardial infarction (MI) has been one of the leading causes of mortality worldwide. MI is characterized by different stages of inflammation of myocardium up to subsequent fibrosis. During the inflammatory process, macrophage population increases and the expression of FR-β on their surfaces are extensive. We studied utility of positron emission tomography/computed tomography (PET/CT) tracer aluminium [18F]fluoride-labelled 1,4,7-triazacyclononane-1,4,7-triacetic acid conjugated folate (Al[18F]F–NOTA–Folate) targeting the FR-β receptor on activated macrophages for the assessment of inflammation in rats with MI. Methods: Surgical ligation of the left anterior descending (LAD) coronary artery irreversibly induced MI, while the sham model was prepared with similar procedures except ligation. Al[18F]F–NOTA–Folate tracer was administered intravenously to perform PET studies at three different time points (3, 7 and 90 days post-MI) using in vivo PET/CT imaging and ex vivo digital autoradiography for both MI and sham groups. Additionally, rat heart cryosections were prepared for histological (hematoxylin-eosin) and immunohistochemical staining with anti-CD68 antibody detecting activated macrophages. Uptake of Al[18F]F–NOTA–Folate was evaluated in the MI region and the remote area in both groups by image analysis. Results: In the MI model, infarcted cardiac areas had higher tracer uptake in ex vivo autoradiography compared to the remote areas or corresponding regions in sham-operated rats. Anti-CD68 immunohistochemistry demonstrated increased macrophage activity in the infarcted areas. The Al[18F]F–NOTA–Folate uptake in the infarcted area and amount of CD68-positive cells correlated positively and significantly. Conclusion: The study suggests that the novel Al[18F]F–NOTA–Folate PET tracer targeting FR-β expressed on activated macrophages is a promising tool for non-invasive imaging of inflammation associated with MI. Further research is required to clarify if this tracer is useful in the diagnostic and prognostic evaluation after MI.