Assessment of mesoporous silica nanoparticle interactions with biofilms by confocal microscopy and surface plasmon resonance
Hellman, Ronja (2022)
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2022051234994
https://urn.fi/URN:NBN:fi-fe2022051234994
Tiivistelmä
Antibiotic resistance is one of the greater threats to worldwide health today. Misuse and overuse of antibiotic agents puts an evolutionary pressure on bacteria, and several different mechanisms of resistance have been detected. The formation of biofilms, clusters of bacteria protected by an extracellular matrix, is one way for bacteria to protect themselves from antimicrobial agents. The ability to penetrate the extracellular matrix is, therefore, essential for the therapeutic efficacy of antibiotics. Nanoparticles hold potential as an anti-biofilm delivery system, as their small size allows them to pass through biological membranes. The morphology and porosity of mesoporous silica particles are promising for drug-loading and specific targeting of biofilms. The shape, size and surface charge of mesoporous silica particles can be tuned, which offers options for drug-loading as well as biocompatibility. One problem with developing anti-biofilm agents is the lack of in vitro models able to represent the complexity of biofilms and their life cycle. Surface plasmon resonance is a relatively new method in biofilm testing but shows great potential as it allows for highly sensitive measurements in real time. In this study, differently shaped and charged mesoporous silica nanoparticles were evaluated for their effect on and penetration of Staphylococcus aureus and Escherichia coli biofilms. Surface plasmon resonance was evaluated as a method for E. coli biofilm testing.
Kokoelmat
- 317 Farmasia [19]