Targeted Sequence Delivery Platform with ROS-Response for the Treatment of Osteoarthritis
Bednjanec, Doroteja (2021)
Bednjanec, Doroteja
2021
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2021061738567
https://urn.fi/URN:NBN:fi-fe2021061738567
Tiivistelmä
Arthritis is a common problem affecting elderly people today. One specific form of arthritis is osteoarthritis, caused by lubrication deficiency at the joint surface. Due to genetics, aging, stress factors, or accidents, the cartilage layer breaks down causing inflammation of the joint. When no cartilage surrounds the bone, neighboring bones scratch each other and cause pain. To prevent and slow down those events, nanomedicine has become a popular field of study and research. The main objective of this research was to develop targeted drug delivery method for treating osteoarthritis using, small nano- and micro- compositions. Another important objective was to find a way for the composition with the lubricated surface, to target damaged tissue. The core of the built nanoparticle was Cerium oxide (CeO2), surrounded by a mesoporous silica nanoparticle (MSN) shell. Using microfluidics, the drug Agomir874 was loaded within core-shell nanoparticles, and then surrounded by the PEO-b-PFMA microparticles. The polymer contained collagen IgG2, for antibody-antigen binding to improve the elasticity of the joint. Finally, the drug 5-ASA was inserted within polymer in order to reduce inflammation in the damaged tissue. As the PEO-b-PFMA particles were diluted, the right solvent was needed to be found in order for the polymer particles to stay stable. In the future, with the help of different imaging techniques, it will be possible to determine how much drug is loaded within the particle and whether the drug loading of the particles is successful.
Kokoelmat
- 3111 Biolääketieteet [11]