Modulation of Human Papilloma Virus transcription by Jagged1 in cervical cancer cell lines
Koivuluoma, Sara (2021)
Koivuluoma, Sara
2021
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2021050328566
https://urn.fi/URN:NBN:fi-fe2021050328566
Tiivistelmä
The Human Papilloma Virus (HPV) is a common sexually transmitted pathogen and the etiological agent for developing cervical cancer (CC). From the high-risk HPVs (hr-HPV), types 16 and 18 are the most prevalent ones. HPV carcinogenesis is driven by the oncoproteins E5, E6, and E7. These proteins' expression is under transcriptional regulation by the HPVs' Long Control Region (LCR). The LCR harbor binding sites for different transcription factors such as Activator Protein 1 (AP-1). The Jagged1 (JAG1) protein is a well-known Notch pathway ligand. Proteolytic processing of JAG1 leads to the release of its intracellular domain (JICD), which potentially could be modulating AP-1 activity.
Here we show that JAG1 is processed in CC-derived cell lines. The overexpression of JAG1 and JICD modulates the HPV16 and HPV18 LCRs in different cervical cell lines. Moreover, we show for the first time that JAG1 can bind to c-Jun, which forms part of the AP-1 complex. Collectively, the results show that JAG1 is involved in controlling the transcription of HPV in cervical cancer through the modulation of the AP-1 complex.
Here we show that JAG1 is processed in CC-derived cell lines. The overexpression of JAG1 and JICD modulates the HPV16 and HPV18 LCRs in different cervical cell lines. Moreover, we show for the first time that JAG1 can bind to c-Jun, which forms part of the AP-1 complex. Collectively, the results show that JAG1 is involved in controlling the transcription of HPV in cervical cancer through the modulation of the AP-1 complex.