Developing a High-Throughput System for monitoring Zebrafish Behaviour
Sarwar, Uruj Fatima (2019)
Sarwar, Uruj Fatima
Åbo Akademi
2019
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe2019060618800
https://urn.fi/URN:NBN:fi-fe2019060618800
Tiivistelmä
The aim of this thesis project was to develop a high-throughput (HT) system for recording the behaviour of zebrafish larvae in response to light stimuli. This was achieved by using a compatible camera, Raspberry Pi Camera (PiCam V2.1) for which drivers exists (PiBoard RPi Zero W V1.1), for imaging zebrafish. A planar white LED copy board A4 light source was used for providing light stimuli. The PiCam was controlled by the PiBoard. Another microcontroller that runs a real-time operating system, Arduino Uno R3, was used to control the light source and synchronise it with the camera controlled via the PiBoard. Zebrafish behaviour was recorded in the form of videos. These videos were converted into image stacks that was further processed with an ImageJ based protocol to identify the percentage of how many zebrafish moved during certain light protocols. Two behaviours were considered for this study, startle response and prepulse inhibition (PPI). A startle response refers to a defensive response to a sudden stimulus in animals and humans. However, in the presence of a weaker stimulus followed by a startling stimulus, the startle response of the organism is reduced, and this is called PPI. The results showed that larval zebrafish exhibited startle response at 4-5 days post fertilisation (dpf) and this was detectable with the developed HT System, however, there was insufficient data to conclude that whether they exhibited PPI or not based on preliminary results using the same system.
Kokoelmat
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